Abstract
Nine hundred and eleven specimens of sputum, 127 of urine, 40 of pleural fluid and 44 others, totalling 1,122.specimens, were cultured in parallel in one Kirchner's medium and on two Löwenstein Jensen's slopes. Myco. tuberculosis was isolated from 135 specimens, 113 were Kirchner positive, and 113 Löwenstein-Jensen positive. The discrepant L.J. positive, Kirchner negative results and vice-versa were mostly confined to smear-negative specimens. Analysis of the positive cultures showed the expected increase in yield by the use of a second L.J. culture, and a much greater increase in yield by using the additional Kirchner culture. These results show a single Kirchner culture to be as efficient as two L.J. cultures, and a little better than a single L.J. culture. Contamination was high with Kirchner's medium, and L.J. culture provided positive cultures more rapidly.
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