Triadic synaptic interactions of large corticothalamic terminals in non-lemniscal thalamic nuclei of the cat auditory system

Abstract

Large corticothalamic (CT) terminals, presumed to originate from cortical layer 5 pyramidal cells, are distributed predominantly in non-specific thalamic nuclei in mammals. In the auditory system, little is known about whether these CT projections participate in the synaptic aggregation referred to as the triad. We studied synaptic interactions of these terminals with neuronal elements in one of the auditory non-lemniscal thalamic nuclei, the dorsal nucleus of the medial geniculate complex (MGC), in cats. After injections of an anterograde tracer in the primary auditory cortex, areas containing labeled large terminals were examined using an electron microscope. It was revealed that a fraction of large CT terminals participated in complicated synaptic arrangements: labeled terminals making synaptic contacts with vesicle-free dendrites, probably of thalamic principal neurons, and/or vesicle-filled neuronal profiles, probably of presynaptic dendrites (PSDs) of interneurons. In reconstructions or even in single sections, we found that these synaptic connections participated in triadic arrangements. Thus, PSDs postsynaptic to the labeled CT terminals were in turn presynaptic to the vesicle-free dendrites.