Abstract
Objective of current study was to determine aflatoxin B1 level in cotton see cake (CSL) rape seed cake (RSC), wheat bran (WB) and compound feed (CF) in 10 districts of Punjab, Pakistan namely Bahawalnagar, Bhakkar, Jhang, Kasur, Khanewal, Layyah, Multan, Muzaffargarh, Rahim Yar Khan and Rajanpur. For this purpose samples of CSC (n=375), RSC (n=100), WB (n=75), CF (n=100) were analyzed at Nutrition Division of Buffalo Research Institute, Pattoki District Kasur. Different samples were extracted, filtered and further screened by using commercially available ELISA Romer kit. The data were analyzed by one-way analysis of variance technique using SPSS software. Highest value of aflatoxin in CSC, RSC, WB and CF was found in districts; Bahawalnagar, Sialkot,Muzaffargarh and Bahawalnagar respectively, while its lowest value in CSC, RSC, WB and CF was found in Muzaffargarh, Multan, Bahawalnagar and Rahim Yar Khan, respectively. Highest infected samples were found in compound feed (21%) followed by cotton seed cake (11.93%), wheat bran (11.71%) and rape seed cake (11.11%) by percentage formula. Based upon the result of recent study toxin binders should be added in processed feed and storage condition of feed ingredients must be improved to minimize the risk of aflatoxicosis.
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