Abstract
Cell-free translation systems have developed significantly over the last 2 decades and improvements in yield have resulted in their use for in vitro protein production in the laboratory. Such cell free systems enable the expression of cytotoxic, regulatory or unstable proteins that cannot be expressed in living cells. We compare the uses of different cell-free translation systems and review recent findings that support the possibility of scaling up and thereby enhancing the industrial application of wheat germ cell free protein expression. Keywords: Cell free biology, in vitro protein expression, scale up of translation, wheat germ extract
Highlights
Cell free protein expression is a rapid and high throughput technology to express proteins from their genes
Bacterial extracts are often unsuitable for translation of RNA because exogenous RNA is rapidly degraded by endogenous nucleases whereas wheat germ cell free translation offers a high throughput system for cell free translation of eukaryotic proteins because of absence of inhibitors
Cell free translation systems including wheat germ lysates are extensively used to discover the molecular mechanisms of protein biosynthesis and in basic sciences
Summary
Cell free protein expression is a rapid and high throughput technology to express proteins from their genes. Cell-free translation systems have developed significantly over the last 2 decades and improvements in yield have resulted in their use for in vitro protein production in the laboratory. We compare the uses of different cell-free translation systems and review recent findings that support the possibility of scaling up and thereby enhancing the industrial application of wheat germ cell free protein expression.
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