Abstract
Counter-current chromatography (CCC), a unique continuous liquid-liquid partition chromatographic technique, has been widely used in the separation and purification of natural products. This technique has made great progress in recent years. In this paper, the different possible separation methods developed in CCC are described. It includes classical CCC, pH-zone-refining, pH-gradient, ion-pairing, multi-channel and two-dimensional CCC, dual-flow, liquid-liquid-liquid three-phase systems, elution-extrusion, gradient elution, powder direct injection, with some words for the various techniques used in pre- and post-CCC procedures, and detection modes. The basic mechanisms of each method are presented and applications are summarized. The future of CCC developments, including its application in protein purification and enrichment, and new platforms, are also discussed. This review paper aims to aid scientists who are interested in the field of CCC research for natural product isolation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
