TREM-2 negatively regulates LPS-mediated inflammatory response in rat bone marrow-derived MSCs.

Abstract

To the best of our knowledge, our previous study demonstrated the expression of triggering receptor expressed on myeloid cells 2 (TREM-2) in human bone marrow mesenchymal stem cells (MSCs) for the first time. However, the inflammation regulatory role of TREM-2 in MSCs remain elusive. The aim of the present study was to investigate the immune regulation and the underlying mechanism of TREM-2 in rat bone marrow MSCs. MSCs were divided into three groups: NullMSCs, TREM-2MSCs, and NormMSCs. TREM-2 was expressed in MSCs at the mRNA and protein level. Following stimulation by lipopolysaccharide (LPS), the gene transcription levels of TREM-2 and inflammatory cytokines were increased. The expression levels of inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), in the TREM-2MSCs lentiviral vector group were significantly downregulated, and the expression of IL-10 was significantly upregulated compared with the controls. Western blot analysis revealed that TREM-2 downregulated the LPS-induced inflammatory response in MSCs, which was probably associated with regulating AKT serine/threonine kinase and p38 mitogen-activated protein kinase downstream signaling proteins. The results of the current study demonstrated that TREM-2 negatively regulates the LPS-mediated inflammatory response in MSCs suggesting that TREM-2 is a potential target of immune regulation in rat MSCs.