Abstract
Fat storing cells (FSC) are the main precursor cell type of liver extracellular matrix synthesis. It is shown here that damage of PC in culture by short-term or permanent exposure to transforming growth factor (TGF)- β 1 increases strongly the mitogenic activity of hepatocyte-conditioned medium for FSC reaching proliferative effects higher than those induced by 10% fetal calf serum. More than 60% of TGF-β-induced hepatocyte-generated mitogenic activity for FSC was abolished by neutralizing anti-TGF-α antibody and by 10 μM tyrphostin 25, a selective TGF-α EGF receptor tyrosine kinase inhibitor. Untreated and TGF-β-pretreated PC cultures showed positive immunofluorescent staining for TGF-α. The results propose potentially a new profibrogenic role of TGF-β via apoptotic and secondary necrotic damage of PC.
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