Treatment of Pimpinella Alpina Molk Improve Oxidative stress and Inhibit Liver Cellular Apoptosis in Rats Following UVB Irradiation: Is there Any Correlation?

Eni Widayati,Taufiqurrachman Nasihun

doi:10.3329/bjms.v17i1.35292

Eni Widayati, Taufiqurrachman Nasihun

Open Access

https://doi.org/10.3329/bjms.v17i1.35292

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Abstract

Objective: The effect of Pimpinella alpina Molk (PaM) in improvement of oxidative stress has been established, but did not for cellular apoptosis. This investigation was conducted to evaluate the effect of PaM on GPx and XO activities, hepatocytes apoptosis and their correlation following UVB radiation.Methods: Forty male rats were assigned into 8 groups: Nor-G, Neg- 7, Neg-15, PaM50-7, PaM100-7, PaM150-7, PaM100-15, and PaM150-15. UVB irradiation was given 10 minutes to each rat daily, blood and liver organ samples were taken in day 8 and day 16 to evaluate all variables.Results: Post Hoc statistical analysis showed that GPx activity in PaM150-15 was not significant lower compared to that of Nor-G, p > 0.05. Conversely, the activity of XO in PaM150-7 was not significant higher compared to that of Nor-G, p > 0.05. Expression of Bax and Caspase3 mRNA in PaM100-15 and PaM150 were comparable to that of Nor-G, p > 0.05. The strong negative correlation (-795, p < 0.01) was occur between GPx and XO. Similarly, there were strong negative correlations (-697 and -715, p < 0.001) between GPx and the expression of Bax and Caspase3 mRNA.Conclusion: PaM treatment with 100 - 150 mg daily dose for 15 days capable of improving oxidative stress marked by increase in GPx activity and decrease in XO activity and inhibit apoptosis characterized by decrease in the expression of Bax and caspase3 mRNA. There were strong negative correlations between antioxidant activity and apoptosis on Sprague male rats after UVB irradiation.Bangladesh Journal of Medical Science Vol.17(1) 2018 p.118-128

Highlights

Pimpinella alpina Molk (PaM), known as Purwoceng, is a medicinal plant growing in Dieng plateau Central Java Indonesia
Considering, degenerative diseases and chronic illness are tightly associated with oxidative stress, daily intakes of flavonoids in midlife and late adulthood are important predictors of subsequent degenerative diseases and disability.[3]
Considering, severe oxidative stress is prerequisite for apoptosis triggered by UVB and posses an important role in aging process and frailty,[8,9] further research is needed to investigate the effect of PaM on antioxidant activity and hepatocyte cells apoptosis, and their relationship following UVB irradiation

Summary

Introduction

Pimpinella alpina Molk (PaM), known as Purwoceng, is a medicinal plant growing in Dieng plateau Central Java Indonesia. Fas/FasL signalling has been largely implicated in liver pathophysiology but the mitochondrial intrinsic pathways are involved in liver homeostasis.[47] In the present study UVB irradiation have been proven induced liver cells (hepatocytes) apoptosis in rats characterized by increase in Bax and caspase[3] mRNA expression in Neg-7 and Neg-15 compared to those of normal and PaM groups. In intrinsic apoptosis, ROS which is produced by UVB is able to damage membrane mitochondrial and activate its protein effectors downstream and activation of caspase[3] later on.[11,49,50] The result of the present study indicated that activities of GPx and XO were increased and decreased respectively following PaM treatment with 100 and 150 mg doses for 15 days. Taken together the results of the present study imply that increase in oxidant invariably followed by decrease in antioxidant and apoptosis after UVB irradiation

Conclusion

Findings

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