Treatment of myocardial infarction by co-injection of rat adipose derived stem cell with basic fibroblast growth factor

Abstract

Objective To This project aimed to discover potential mechanism of treating myocardial infarction by transplantation of adipose-derived stem cells (ADSC) with basic fibroblast growth factor(bFGF).Methods ADSC were isolated from the fat tissue.These cells were cultured and induced to differentiate to the adipogenic cells,which were identified by rows stem cell adipogenic and osteogenic differentiation.Myocardial infarction (MI) model were conducted in 50 SD rats,and rats were randomized into 4 groups with the inclusion criterion of EF (ejection fraction) ＜40％ by echocardiography 1 week after MI (PBS group,bFGF group,PBS+ADSC group,ADSC+bFGF group).bFGF was co-injected with ADSC into the left ventricular wall of MI rats.After 4 weeks,MI size,left ventricular wall thickness,MI parts micro-vessel density,ADSC differentiation at the MI site were detected and cardiac function measured by echocardiography.Immunohistochemistry and immunofluorescence were used to evaluate possible mechanism.Results ADSC were successfully isolated and most ADSC showed positive and CD90 and CD29 and negative,CD45 and CD34 by flow cytometry.ADSC could be differentiated into adipogenic,and osteogenic cells.PBS + ADSC group and ADSC + bFGF group showed statistically significant differences of angiogenesis,ventricular wall thickness MI size,MI regional capacity compared to PBS groups,especially ADSC+bFGF group (P＜0.01).After 4 weeks of ADSC transplantation into rats,the cells could differentiate into positive cTnT,SMA positive cells and vWAg positive cells.Cardiac function test results showed that PBS+ADSC group had the same ability of repairment as ADSC+bFGF group.Conclusion Treatment of cotransplanting ADSC with bFGF can promote the regeneration of infarcted myocardium and blood vessels,and improve the cardiac function. Key words: Myocardial infarction; Adipose derived stem cell; Basic fibroblast growth factor