Abstract
Isolated pistils of distylous buckwheat (Fagopyrum esculentum Moench) were treated with protease inhibitors (PMSF, pepstatin A, and antipain). Pistils were cross- or self- pollinated, and growth of pollen tubes was observed under a fluorescence microscope. Treatments with all inhibitors suppressed inhibition of self-pollen tube growth, suggesting that activity of proteases is involved in rejection of self-pollen during the SI response.
Highlights
Self-incompatibility (SI) is an outbreeding mechanism which enables plants to discriminate between self and non-self pollen grains
We describe effects of protease inhibitors on the self-incompatibility response in order to further “dissect” the SI response in buckwheat and analyze the possible role of proteases in this complex process
We examined the effects of different protease inhibitors to establish possible protease involvement in the SI response of buckwheat
Summary
Self-incompatibility (SI) is an outbreeding mechanism which enables plants to discriminate between self and non-self pollen grains It is broadly distributed among the flowering plants: more than half of all flowering plant species display some of the SI types. The heteromorphic SI system is characterized by different positions of stigmas and anthers in dimorphic or trimorphic flowers (de Nettancourt, 1997) It is rare and occurs in 24 families of flowering plants. Among plants of this type, common buckwheat (Fagopyrum esculentum Moench) is of the greatest economical importance. It posseses two flower morphs: pin (with long pistil and short anthers) and thrum (with short pistil and long anthers), which are distributed among the population. Cross and self-pollinated, were treated with three different protease inhibitors: the serine protease inhibitor PMSF, the aspartic protease inhibitor pepstatin A, and the cysteine protease inhibitor antipain
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