Treatment of gregarines sporozoites in shrimp with allicin an herbal preparation and control intermediate hosts with copper sulphate

Abstract

Purpose: The purpose of study is to evaluate Allicin supplementation to tackle Gregarines Sporozoites infection in shrimp. Parasitic infections and associated diseases are a significant risk factor in aquaculture productivity, marine, and freshwater. Apart from bacterial and viral infections, aquatic animals are prone to protozoa infection. As the most common parasitic protozoa infection in marine shrimp, Gregarines reported a significantly high infection frequency, i.e., up to 90%. Penaeid shrimp are infected by at least three genera of gregarine (Protozoa, Apicomplexa). Method: Here, in this study, allicin an organo-sulfur compound used to treat gregarines infections in shrimps. Here, garlic paste containing allicin was incorporated in the feed for 10 days at 10, 20, 30, 40, and 50 gm/Kg concentration twice in a day. Microscopic analysis was carried out to evaluate effect of Allicin with copper sulphate supplemented in shrimp feed. Results: Study reports here, shrimps fed with garlic paste as the major source of allicin shown a significant reduction in gregarines. As a control, we used turmeric in shrimp feed given at 5, 10, 15 and 20 g/Kg in two times a day for 10 days. Here, we also incorporated a second dose of allicin along with copper sulphate at 0.1, 0.2, 0.3, 0.4 and 0.5 ppm concentrations to minimize secondary infection. Conclusion: We report here, administration of garlic paste to shrimp significantly reduces gregarines in shrimps.