Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networks

Abstract

Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood. In this study, GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area were significantly inhibited in early stages after GA3 treatment. Microscopic examination showed that GA3 treatment changed the cortex thickness, the pericycle diameter, and cell size in main root. Interestingly, exogenous GA3 increased the quantity of lateral root primordia (LRP), but LR number decreased in this period. Moreover, the content of GAs, auxin and abscisic acid in root was altered. RNA-seq results revealed that application of GA3 not only changed the expression of genes in GA biosynthesis pathway, including GA20ox and GA2ox, but also the GA regulation genes and signalling pathway genes. The changes in expression of gene concerning other hormones were also detected. In addition, GA3 altered cell wall biogenesis and degradation genes which might be related to the changes of root morphology. In response to increased GA3, 103 transcription factors were detected. Thus, exogenous GA3 changed the content of hormones in roots and affected the root development by regulating the expression of respective genes.