Abstract
Abstract Computer simulation of the full time course of an enzymatic reaction may be used to help define certain characteristics of the mechanism of reaction. In particular, such studies may be the best way to approach the kinetic properties of those enzymes which show hysteretic behavior. Furthermore, full time course studies may be used to differentiate between various mechanisms involving two substrates. For example, it is shown that different time courses may be obtained for a mechanism assuming ordered addition of substrates relative to one assuming a random addition of substrates. It is pointed out that the use of full time course studies may be applicable to a large number of mechanisms as a routine kinetic method of great utility for approaching the definition of such mechanisms. After a mechanism for an enzymatic reaction has been assumed, the full time course computer simulation program discussed here can also be used as a data-fitting program to obtain kinetic parameters for both the forward and reverse reactions.
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