Abstract
We evaluated the effects of Lycium barbarum polysaccharides LBP) on D-galactose aging model mouse, and explored its possible mechanism. Kunming mice were randomly divided into the control group, the model group, the high-dose LBP group, and the low-dose LBP group. Except the control group, D-galactose was used for modelling. The drug was administrated when modelling. Mouse behavioural, learning and memory changes were observed, and the contents of lipid peroxidation (LPO), lipofuscin (LF) and monoamine oxidase B (MAO-B) in mouse brain tissue and the weight of immune organs were measured after 6 weeks. Compared with the control group, mouse weight gain in the model group reduced significantly. Compared with model group, after mice drank LBP, the times of electric shock was less than aging mice (in which, the high-dose LBP group, P<0.05), and electric shock incubation period was longer (P<0.01). On Day 45 after modelling and drug administration, the contents of LPO, LF and MAO-B in mouse brain tissue in the model group increased significantly, while those in the drug administration groups decreased significantly. The thymus index in the aging model group decreased significantly; the thymus index and the spleen index in the high-dose LBP group and the low-dose LBP group rebounded significantly (P<0.01). We concluded that LBP has an anti-aging effect on D-galactose induced aging model mouse, and its mechanism may be related with the alleviation of glucose metabolism disorder and the resistance of the generation of lipid peroxide and other substances, which damage cell membrane lipid.
Highlights
Barbary wolfberry fruit is the dried ripe fruit of Lycium barbarum L. (State Pharmacopeia Committee., 2010), with immunity enhancing, liver protecting, blood sugar lowering, anti-tumour, blood pressure lowering, antioxidant, and anti-aging effects (Kim et al, 1997; Kim et al, 2000; Peng & Tian., 2001; Leung et al, 2001; Toyoda-Ono et al, 2004) and other functions, and is a traditional precious Chinese herbal medicine of long history
This paper starts from the metabolism theory of aging, and uses D-galactose aging mouse model (Miao., 2002) to study the anti-aging effect of lycium barbarum polysaccharides (LBP) and to preliminarily explore its anti-aging mechanism
The results show that after the mice drank LBP, the number of electric shocks are less than that for aging mice, and the electric shock incubation period prolongs (P
Summary
Barbary wolfberry fruit is the dried ripe fruit of Lycium barbarum L. (State Pharmacopeia Committee., 2010), with immunity enhancing, liver protecting, blood sugar lowering, anti-tumour, blood pressure lowering, antioxidant, and anti-aging effects (Kim et al, 1997; Kim et al, 2000; Peng & Tian., 2001; Leung et al, 2001; Toyoda-Ono et al, 2004) and other functions, and is a traditional precious Chinese herbal medicine of long history. After the behaviour experiment and at 45 days after modelling and drug administration, the mice were decapitated, and the whole brain (cerebellum removed) was taken out quickly and separated at the mid sagittal suture, one half for the determination of LPO and LF contents, and the other half for the determination of MAO-B activity. We took appropriate amount of fresh brain tissue, added 10 times of volume of pre-cooled 0.2mol/L phosphate buffer (pH 7.4), homogenised in a glass homogeniser in ice bath, and made ultrasonic extraction for 20 seconds twice and at an interval of 30 seconds, centrifuge at 1000×g and 0°C for 10 minutes. We added 0.3ml 60% perchloric acid to terminate the reaction, added 4.0ml cyclohexane for mixing and extraction, centrifuged at 3000r/min for 10 minutes, and took the supernatant to measure the A value at 242nm. Mice were weighed before being killed, the thymus and spleen were weighed on an electronic tissue scale after cleaning, and the thymus index and spleen index were calculated
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