Abstract
Chronic kidney disease (CKD) is characterized by structural abnormalities and the progressive loss of kidney function. Extracellular vesicles (EVs) from human umbilical cord tissue (hUCT)-derived mesenchymal stem cells (MSCs) and expanded human umbilical cord blood (hUCB)-derived CD133+ cells (eCD133+) maintain the characteristics of the parent cells, providing a new form of cell-free treatment. We evaluated the effects of EVs from hUCT-derived MSCs and hUCB-derived CD133+ cells on rats with CDK induced by an adenine-enriched diet. EVs were isolated by ultracentrifugation and characterized by nanoparticle tracking analysis (NTA) and electron microscopy. The animals were randomized and divided into the MSC-EV group, eEPC-EV group and control group. Infusions occurred on the seventh and 14th days after CKD induction. Evaluations of kidney function were carried out by biochemical and histological analyses. Intense labeling of the α-SMA protein was observed when comparing the control with MSC-EVs. In both groups treated with EVs, a significant increase in serum albumin was observed, and the increase in cystatin C was inhibited. The results indicated improvements in renal function in CKD, demonstrating the therapeutic potential of EVs derived from MSCs and eCD133+ cells and suggesting the possibility that in the future, more than one type of EV will be used concurrently.
Highlights
We evaluated the effects of extracellular vesicles (EVs) from human umbilical cord tissue-derived mesenchymal stem cells (MSCs) and expanded human umbilical cord blood-derived CD133+
Immunophenotypic characterization of CD133+ cells was performed after expansion and showed low expression levels of CD133 and CD 34 compared to the immunophenotypic profile of purified CD133+ (Figure 2b)
We observed a significant increase in α-smooth muscle actin (α-SMA) expression in the control group compared to the MSC-EV group
Summary
Dayane Mayumi Miyasaki 1,2, Alexandra Cristina Senegaglia 1,2 , Sérgio Adriane Bezerra de Moura 3 , Amanda Leitolis 2,4 , Luiz Guilherme Achcar Capriglione 1,2, Letícia Fracaro 1,2 , Lidiane Maria Boldrini Leite 1,2 , Paulo Henrique Utumi 1,2 , Felipe Yukio Ishikawa Fragoso 1,2 , Fernando Meyer 1 , Alejandro Correa 2,4,*,†.
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