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Abstract
Two experiments investigated the potential of Phanerochaete chrysosporium ligninase to delignify straw and thereby improve its digestibility for ruminants. In Experiment 1, milled straw was added to media containing the fungus P. chrysosporium, with or without addition of extra ligninase, and the effects of treatments on chemical composition and digestibility in vitro were measured after 5 days. There was no effect ( P > 0.05) of ligninase on either digestibility or chemical composition as measured by Van Soest methods. Ligninase activity was assessed at days 3, 4 and 5; no activity could be detected. Experiment 2 showed that water-soluble component(s) from barley straw inhibited ligninase activity; 10 μl of water extract completely inactivated 5.0 units of ligninase in 1.0 ml of solution. However, sodium dodecyl sulphate (SDS)-gel electrophoresis in Experiment 2 showed that the enzyme as a protein had not been degraded.
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