Abstract

Abstract The treatment efficiency of two-stage and two-phase (TSTP) anaerobic process composed of hydrolytic acidification reactor, first-order and second-order external circulation (EC) anaerobic reactors were investigated and the characteristics of microbiological population composition in different reactors were also studied through scanning electron microscope. Results showed that by seeding anaerobic granular sludge, controlling start-up volume loading rate and the type of loading rate increasing, hydrolytic acidification reactor quickly started up in 34 d and formed stable ethanol-type fermentation. Under the conditions of organic loading rate (OLR) = 46.78 kgCOD/m3 d, hydraulic retention time (HRT) = 5.62 h, chemical oxygen demand (COD) removal rate of first-order EC reactor was 93.2%, effluent COD was 700 mg/L, and that of second-order EC reactor was 83.3% and 110 mg/L. Furthermore, TSTP process presented strong anti-shock loading capability. Dominant bacteria were different in different reactors....

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