Traveling waves in yeast extract and in cultures of Dictyostelium discoideum

Abstract

Biological self-organization was investigated in a biochemical and a cellular system: yeast extract and cultures of the slime mold Dictyostelium discoideum. In both systems traveling reaction-diffusion waves occur in response to oscillatory reactions. Glycolytic degradation of sugar in a yeast extract leads to the spontaneous formation of NADH and proton waves. Manipulation of the adenine nucleotide pool by addition of purified plasma membrane ATPase favors the formation of both reaction-diffusion waves and phase waves. The results indicate that the energy charge has an important impact for the dynamics of glycolytic patterns. When affecting the lower part of glycolysis by pyruvate addition the frequency of wave generation was increased with concomitant formation of rotating NADH and proton spirals. During morphogenesis of the cellular system Dictyostelium discoideum, circular and spiral shaped aggregation patterns of motile amoeboid cells form in response to traveling cAMP waves. Velocity analysis of the cell movements reveals that the cAMP waves guide the cells towards the site of wave initiation along optimized trajectories. The minimization of aggregation paths is based on a mechanism exploiting general properties of excitation waves. The resulting aggregation territories are reminiscent of Voronoi diagrams.