Abstract
Leukaemic cells from rats with a lymphoid (HRL) or myeloid (SAL) leukaemia were labelled with 125IUDR and injected i.v. into either normal or leukaemic syngeneic recipients. The fate of the injected cells was studied in terms of the radioactivity in various tissues at various times up to 24 h later. In normal animals the leukaemia cells were destroyed rapidly in the reticulo-endothelial (RE) system; immediately after injection most recoverable activity was in the lung, with smaller amounts in the blood, spleen and liver but by 24 h only 20-30% of the injected activity could be recovered. In leukaemic recipients with high numbers of blasts in the blood the amount of activity recoverable from the lungs and bone-marrow was markedly reduced, while that in the blood was doubled. Nonetheless, the overall rate at which radioactivity was eliminated was not significantly different from that found in normal rats, in spite of the fact that the RE system was extensively infiltrated by leukaemia cells.
Highlights
In leukaemic recipients with high numbers of blasts in the blood the amount of activity recoverable from the lungs and bone-marrow was markedly reduced, while that in the blood was doubled
The increased suspended in TC 199 (Wellcome). These resistance of the lungs to i.v. challenge with tumour cells which is observed when rodents are treated with Corynebacterium parvrum
The fate and rate of death of i.v.-inoculated leukaemia were incubated for 1 h with 0-1,C/ml at 38°C, washed 3 times centrifuging for 5 min at 240 g and suspended at concentrations of 108 cells/ml (Hall and Smith, 1970)
Summary
Rats-.Pure line, barrier-maintained, male rats were given 150 ,uC 125IUDR i.v. at hooded and female August rats, 8-10 weeks 0 and 8 h. (Wrathmell, 1976), which arose spontaneously Heat-killed cells.-Labelled cells were in a female August rat in 1968, was main- killed by heating in a 55°C water bath for tained by blood passage of 105 spleen or 45 min. They were washed before transblood cells every 7 days. In every case the following tissues were removed: lung, liver, spleen, small gut (flushed of contents), thymus, lymph nodes (mesenteric, mediastinal, superficial and deep cervical), bone marrow (as femur), and blood taken by cardiac puncture. When 3H-thymidine-labelled cells had been injected, the lung, liver and spleen were removed from recipient rats, fixed in other than those routinely sampled. At 4 h the liver showed a scattering of label with few intact labelled
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