Abstract

Transtracheal puncture has long been known as a safe, low-cost procedure. However, with the advent of bronchoscopy, it has largely been forgotten. Two researchers have suggested the use of α-amylase activity to diagnose salivary aspiration, but the normal values of this enzyme in tracheobronchial secretions are unknown. We aimed to define the normal values of α-amylase activity in tracheobronchial secretions and verify the rate of major complications of transtracheal puncture. From October 2009 to June 2011, we prospectively evaluated 118 patients without clinical or radiological signs of salivary aspiration who underwent transtracheal puncture before bronchoscopy. The patients were sedated with a solution of lidocaine and diazepam until they reached a Ramsay sedation score of 2 or 3. We then cleaned the cervical region and anesthetized the superficial planes with lidocaine. Next, we injected 10 mL of 2% lidocaine into the tracheobronchial tree. Finally, we injected 10 mL of normal saline into the tracheobronchial tree and immediately aspirated the saline with maximum vacuum pressure to collect samples for measurement of the α-amylase level. The α-amylase level mean ± SE, median, and range were 1914 ± 240, 1056, and 24-10,000 IU/L, respectively. No major complications (peripheral desaturation, subcutaneous emphysema, cardiac arrhythmia, or hemoptysis) occurred among 118 patients who underwent this procedure. Transtracheal aspiration is a safe, low-cost procedure. We herein define for the first time the normal α-amylase levels in the tracheobronchial secretions of humans.

Highlights

  • Clarke et al [1] and Nandapalan et al [2,3] have suggested the use of a-amylase activity as a marker of saliva aspiration

  • We prospectively evaluated 118 patients with indications for diagnostic bronchoscopy to define the levels of a-amylase activity in tracheobronchial secretions in patients without risk factors for aspiration and to study the rate of major complications of transtracheal puncture

  • The objectives of the present study were to define the normal values of a-amylase activity in patients without risk of salivary aspiration using transtracheal puncture to collect specimens from the respiratory system and to determine the rate of major complications of this procedure

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Summary

Introduction

Clarke et al [1] and Nandapalan et al [2,3] have suggested the use of a-amylase activity as a marker of saliva aspiration. We prospectively evaluated 118 patients with indications for diagnostic bronchoscopy to define the levels of a-amylase activity in tracheobronchial secretions in patients without risk factors for aspiration and to study the rate of major complications of transtracheal puncture. Pecora [4] was the first to use transtracheal puncture to collect specimens for bacteriologic examination in patients with lung infections. His idea came from a publication by Bonica [5] in 1948 about intratracheal anesthesia. The objectives of the present study were to define the normal values of a-amylase activity in patients without risk of salivary aspiration using transtracheal puncture to collect specimens from the respiratory system and to determine the rate of major complications of this procedure. We evaluated patients undergoing examinations for subcutaneous emphysema, peripheral desaturation, hemoptysis, and cardiac arrhythmias

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