Abstract
Abstract The discovery of mobile genetic elements and their association with ‘spontaneous’ mutations in nature has led to novel ways of cloning genes, of germline transformation, and has added methods for creating certain types of mutations. To date, the P element is the most exploited transposable element in Drosophila, but there are many others, several of which have been characterized sufficiently to allow similar applications with perhaps only a little more effort. For example, the hobo element has been used for both transformation and gene tagging, the IS elements have been used for mutagenesis and gene tagging, and mariner has been used for transformation. These other mobile elements may have properties which, in the future, make them better for certain applications, such as target site specificity, efficiency of movement, or homologous target integration (gene replacement). But, this chapter focuses on some of the common applications of the P transposable element technology for creating useful mutations. Specifically it focuses on:
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
