Transposon-mediated directed mutation controlled by DNA binding proteins in Escherichia coli.

Milton H Saier,Zhongge Zhang

doi:10.3389/fmicb.2014.00390

Abstract

Transposon-mediated directed mutation controlled by DNA binding proteins in Escherichia coli.

Highlights

It is a basic principle of genetics that the likelihood of a particular mutation occurring is independent of its phenotypic consequences
The concept of directed mutation, defined as genetic change that is induced by the stress conditions that the mutation relieves (Cairns et al, 1988), challenges this principle (Foster, 1999; Rosenberg, 2001; Wright, 2004)
Directed mutation has been defined as genetic change that is induced by the stress conditions that the mutation relieves, but until recently, in no case had such a mechanism been established

Summary

INTRODUCTION

It is a basic principle of genetics that the likelihood of a particular mutation occurring is independent of its phenotypic consequences. The E. coli glycerol (glp) regulon consists of five operons, two of which (glpFK and glpD) are required for aerobic growth on glycerol (Lin, 1976). Both operons are subject to negative control by the DNAbinding glp regulon repressor, GlpR (Zeng et al, 1996), which binds glycerol-3phosphate, the inducer of the glp regulon. When crp cells were incubated on solid glycerol minimal medium, Glp+ colonies appeared. Colonies first appeared after about 3 days (Figure 1B) wild type (wt) and crp Glp+ E. coli cells formed visible colonies in

Saier and Zhang

CONCLUSIONS AND PERSPECTIVE