Abstract
Transposon-mediated sequencing is an effective method for obtaining full-length high-quality DNA sequence. This method can be applied to the finishing stages of bacterial artificial chromosome (BAC) sequencing, allowing the user to expand a finishing repertoire of custom oligonucleotide sequencing, alternative chemistry sequencing, polymerase chain reaction (PCR), as well as other standard tools for acquiring high-quality data in low-coverage or low-quality regions. In addition to BAC subclones, transposon-mediated sequencing can be applied to cDNA or PCR products subcloned into vectors.
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