Transport-related modulation of the membrane properties of toad urinary bladder epithelium

Abstract

Impedance analysis and transepithelial electrical measurements were used to assess the effects of the apical membrane Na + channel blocker amiloride and anion replacement on the apical and basolateral membrane conductances and areas of the toad urinary bladder ( Bufo marinus). Mucosal amiloride addition decreased both apical and basolateral membrane conductances ( G a and G bl, respectively) with no change in membrane capacitances ( C a and C bl). Consequently, the specific conductances of these membranes decreased without significant changes in membrane area. Following amiloride removal, an increase was obtained in the steady-state rate of sodium transport compared to values before amiloride addition. This increase was independent of the initial transport rate, suggesting activation of a quiescent pool of apical sodium channels. Chloride replacement by acetate or gluconate had no significant effects on apical or basolateral membrane capacitances. The effects of these replacements on membrane conductances depended on the anion species. Gluconate (which induces cell shrinkage) decreased both membrane conductances. In contrast, acetate (which induces cell swelling) increased G a and had no effect on G bl. The increase in the apical membrane conductance was due to an increase in the amiloride-sensitive Na + conductance of this membrane. In summary, mucosal amiloride addition or chloride replacements led to changes in membrane conductances without significant effects on net membrane areas.