Abstract
A method is described for isolating apical plasma membrane vesicles from fetal alveolar type II cells. The procedure yields purified apical membranes which are enriched 24-fold with the brush-border enzyme marker, alkaline phosphatase. Contamination of this fraction by basolateral membranes and organelles is minimal. Evidence for transport of Na + into an intravesicular space is demonstrated by: (1) time-dependent uptake of Na + with release of accumulated Na + by treatment with detergent; (2) a linear inverse correlation between Na + uptake and medium osmolarity. In addition, Na + uptake is shown to be anion dependent (SCN − > Cl − > gluconate −) and sensitive to amiloride inhibition at a concentration of 1 mM.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Biomembranes
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