Abstract
Evidence for the existence of mediated transport of pyruvate and lactate in isolated mitochondria of Saccharomyces cerevisiae is presented. 1. 1. The mitochondrial oxidation of pyruvate is specifically inhibited by the monocarboxylic oxoacids α-ketovalerate, α-ketoisocaproate and by α-cyano-3-hydroxycinnamate, while pyruvate and malate dehydrogenases activities are not inhibited. 2. 2. The stimulation of the mitochondrial oxidations of succinate, α-ketoglutarate and citrate by pyruvate are also inhibited by α-cyano-3-hydroxycinnamate. 3. 3. The [ 14 C]pyruvate uptake by yeast mitochondria follows saturation kinetics and is completely inhibited by α-cyano-3-hydroxycinnamate. 4. 4. Large amplitude passive swellings of mitochondria of the wild type and of cytoplasmic rho − and rho − n mutants are induced by isoosmotic ammonium pyruvate and lactate. These pH-dependent swellings are inhibited by α-cyano-3-hydroxycinnamate suggesting that the carrier system is not coded by mitochondrial DNA.
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