Transport of mono- and divalent cations across chloroplast membranes mediated by the ionophore A23187

Abstract

Effects of the ionophore A23187 on isolated broken and intact chloroplasts in the pH range of 6.2 to 7.6 have been studied. In both types of chloroplasts, uncoupling of photosynthetic electron transport by A23187 (6–10 μ m) was mediated either by Mg 2+ or—in the absence of divalent cations (i.e., when EDTA was added to the medium)—by high concentrations of Na +, but not of K + ions. At increased concentrations of the ionophore (above about 10 μ m) and high pH (7.2 to 7.6), uncoupling in broken chloroplasts was also mediated by K + ions. The inhibition of the energy-dependent slow decline of chlorophyll fluorescence in intact chloroplasts by the ionophore (which denotes uncoupling) is reversed by EDTA in the presence of K +, but not of Na + ions. In 3-(3′,4′-dichlorophenyl)1,1-dimethylurea-poisoned intact chloroplasts, the yield of variable chlorophyll fluorescence is lowered by A23187 + EDTA and increased again by addition of NaCl or KCl. Chlorophyll fluorescence spectra at 77 °K of intact chloroplasts incubated with A23187 + EDTA indicated that the distribution of excitation energy had changed in favor of photosystem I, as expected from a depletion of Mg 2+. This change was reversed by MgCl 2+, KCl, or NaCl. From a comparison of low-temperature fluorescence spectra of broken and intact chloroplasts at different levels of Mg 2+ in the medium, the concentration of free Mg 2+ in the stroma of the intact chloroplasts at pH 7.6 in the dark was estimated at 1 to 4 m m. The results show that in chloroplasts the specificity of A23187 for divalent cations is limited. In the presence of EDTA, the ionophore mediates fast Na + H + exchange across thylakoid membranes, whereas K + is transferred much less efficiently. Both Na + and K + ions seem to be transported readily across the chloroplast envelope by the action of the ionophore, leading to an exchange of Mg 2+ for monovalent cations at the thylakoid membrane surfaces in intact chloroplasts.