Abstract
We used carefully defined heme-hemopexin complexes to investigate the role of hemopexin in the catabolism of heme in vivo. Uptake of rabbit [ 59Fe]heme-[ 125I]hemopexin by rat liver was rapid. The liver-associated 125I reached a maximum 5 minutes after injection, nearly 7-fold higher than apo-hemopexin, whereas liver-associated 59Fe increased with time. This together with an inverse relationship of [ 125I]hemopexin in the liver and serum during the course of heme transport suggests that hemopexin was released from the liver back to the circulation. Saturation of uptake with heme-hemopexin, reaching about 170 pmol [ 125I]hemopexin (gm liver) −1 5 minutes after injection of 11 nmol, indicates a receptor-mediated process. We conclude that hemopexin delivers heme to the liver via interaction with a finite number of receptors and returns to the circulation.
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