Abstract
Conflicting reports exist concerning the presence of a Na +-coupled amino acid transport system in cultured endothelial cells. We have employed a non-metabolizable analog, 2-aminoisobutyric acid (AIB), to investigate the activity of Na +-dependent amino acid transport in cultured human umbilical vein endothelial cells (HUVEC). We found a pronounced saturable, Na +-dependent component of AIB uptake in ‘fresh’ (non-starved) HUVEC. The Na +-dependent component accounted for 78% of total AIB uptake with a high sensitivity to external Na +. The accumulation of AIB was inhibited by ouabain preincubation, consistent with the energetics of Na +-coupled transport. Amiloride, an epithelial Na + channel blocker, also inhibited AIB transport at high concentration. The results strongly support the presence of a Na +-coupled transport system of amino acid in HUVEC.
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