Abstract
SummarySeveral aspects of the behavior of antibodies at substrate-supported planar membranes have been characterized with quantitative fluorescence microscopy: (1) very slow rotational mobilities of an anti-dinitrophenyl monoclonal antibody associated with solid-like phospholipid Langmuir-Blodgett monolayers have been measured with polarized fluorescence photobleaching recovery; (2) the equilibrium and kinetics of an anti-dinitrophenyl Fab at phospholipid Langmuir-Blodgett monolayers have been characterized with total internal reflection fluorescence microscopy; and (3) the apparent association constants of a panel of anti-dinitrophenyl mouse IgGl antibodies with substrate-supported planar membranes containing purified and reconstituted mouse IgG Fc receptors have been measured with total internal reflection fluorescence microscopy.
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