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Abstract
The values of permeability coefficients to water molecules and cryoprotectants are demanded to select the optimal duration of exposure of cells in cryoprotective media at the stage of their preparation for cryopreservation, as well as to find optimal cooling and warming rates during the freeze-thawing of cell suspensions. The necessary numerical values of such cell parameters as the osmotically inactive volume α and the surface-area-to-volume ratio γ were obtained for the analytical evaluation of the permeability coefficients of the PK-15 cells’ plasma membranes using physico-mathematical modelling. The permeability coefficients kp of the plasma membranes of PK-15 cells to 1,2-PD, EG, DMSO and glycerol cryoprotectants molecules, as well as the filtration coefficients Lp to water molecules at temperatures of 25, 15 and 5°C were determined by approximating the experimental data of the change in relative volume of cells on exposure time in the studied solutions by theoretical curves calculated on the basis of physical and mathematical model of passive transport of water and permeable substances under the condition of their maximum coincidence. The value of the activation energy of the transmembrane transfer of molecules of these substances is calculated
Highlights
The values of permeability coefficients to water molecules and cryoprotectants are demanded to select the optimal duration of exposure of cells in cryoprotective media at the stage of their preparation for cryopreservation, as well as to find optimal cooling and warming rates during the freeze-thawing of cell suspensions
At all the stages of low-temperature preservation technological cycle, the cells are repeatedly subjected to changes in osmolarity of extracellular environment, resulting in significant differences in osmotic pressure on a cell membrane
The determination of permeability coefficients to water molecules and cryoprotectants is needed to select the optimal duration of cell exposure in cryoprotective media to prepare them for cryopreservation, as well as to find optimal cooling and warming rates
Summary
The values of permeability coefficients to water molecules and cryoprotectants are demanded to select the optimal duration of exposure of cells in cryoprotective media at the stage of their preparation for cryopreservation, as well as to find optimal cooling and warming rates during the freeze-thawing of cell suspensions. The response of cells to extreme factors, which inevitably accompany the cryopreservation is determined, in particular, by the transport and mechanical properties of cell membranes characteristic of each cell type In this regard, the determination of permeability coefficients to water molecules and cryoprotectants is needed to select the optimal duration of cell exposure in cryoprotective media to prepare them for cryopreservation, as well as to find optimal cooling and warming rates.
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