Abstract
Nude mouse human thymus transplant model: Fresh or cryopreserved and thawed human thymus slices were transplanted subcutaneously into recipient nude mice. Nude mice subsequently produced mouse CD3+ CD4+ T-cells.
Highlights
We show that slices of human thymus tissue that have been frozen and thawed can induce and support T-cell development when transplanted into nude mice
Slices of thymus tissue are transplanted into the thigh, after a 2- to 3-week culture period to deplete thymocytes
We decided to use a nude mouse model of human thymus transplantation [6] to test if slices of human thymus tissue that have been frozen and thawed could still induce and support T-cell development
Summary
We show that slices of human thymus tissue that have been frozen and thawed can induce and support T-cell development when transplanted into nude mice. This discarded thymus tissue can be transplanted into athymic infants [2,3,4]. Slices of thymus tissue are transplanted into the thigh, after a 2- to 3-week culture period to deplete thymocytes.
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