Abstract
By means of collagenase digestion and the Ficoll gradient separation technique, viable islets of Langerhans were isolated from canine pancreas. These islets were transplanted into the livers of diabetic dogs. Experimental diabetes was induced by subtotal pancreatectomy and two injections of 25 mg streptozotocin/kg body wt. Five recipients received islets from weakly histocompatible donors and were immunosuppressed by administration of azathioprine and anti-thymocyte serum. Diabetes was ameliorated over a period of 1 year. Glucose tolerance test curves and immunoreactive insulin concentrations in the portal and superior vena cava were significantly impaired in comparison to the diabetic control dogs. No signs of portal hypertension or of disturbances of liver function were observed in recipient dogs during the duration of the experiment.
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