Transplantation of cultured human spinal cord cells into the rat motor cortex: use of Phaseolus vulgaris leucoagglutinin as a cell marker

Abstract

Successful transplantations have been made of cultured explants of human fetal spinal cord into surgically created cavities in the motor cortical area of non-immunosuppressed young adult rats. The cultured cells were marked by brief incubation with Phaseolus vulgaris leucoagglutinin (PHA) just prior to transplantation. Following sacrifice of the rats 1.5 months later, PHA immunohistochemistry clearly outlined the demarcation zone of the explants. The transplanted neurons possessed long, somewhat tortuous fibers with occasional varicosities, as well as some thick processes. These findings extend our previous studies in which it was shown that cultured human fetal adrenal medulla and sympathetic ganglia cells could be successfully transplanted to non-immunosuppressed rat brain. They also suggest that PHA may be a valuable marker for transplanted cells at least for 1.5 months post-transplantation.