Abstract
Ischemia-reperfusion injury is an important contributor to acute kidney injury and a major factor affecting early functional recovery after kidney transplantation. We conducted this experiment to investigate the protective effect of induced multipotent stem cell transplantation on renal ischemia-reperfusion injury. Forty rabbits were divided into four groups of 10 rabbits each. Thirty rabbits were used to establish the renal ischemia-reperfusion injury model, and ten rabbits served as the model group and were not treated. Among the 30 rabbits with renal ischemia-reperfusion injury, 10 rabbits were treated with induced peripheral blood mononuclear cells (PBMCs), and 10 other rabbits were treated with noninduced PBMCs. After three weekly treatments, the serum creatinine levels, urea nitrogen levels and urine protein concentrations were quantified. The kidneys were stained with hematoxylin-eosin (HE), periodic acid-Schiff (PAS) and Masson's trichrome and then sent for commercial metabolomic testing. The kidneys of the rabbits in the model group showed different degrees of pathological changes, and the recovery of renal function was observed in the group treated with induced cells. The results indicate that PBMCs differentiate into multipotent stem cells after induction and exert a therapeutic effect on renal ischemia-reperfusion injury.
Highlights
Clinical acute kidney injury, which has a very high incidence, might be caused many factors, and ischemia-reperfusion injury is the most common of these factors [1]
We investigated the protective effect of induced multipotent stem cell transplantation on renal ischemia-reperfusion injury in rabbits, and our results provide experimental evidence and ideas for clinical treatment
The expression of NANOG, OCT4 and SOX2 was significantly increased in peripheral blood mononuclear cells (PBMCs) stimulated with chicken egg white extract, and the expression of the somatic cell gene LMNA was decreased (Fig 1D), which indicated that the cells differentiated into multipotent cells
Summary
Clinical acute kidney injury, which has a very high incidence, might be caused many factors, and ischemia-reperfusion injury is the most common of these factors [1]. The main pathological manifestation of acute kidney injury is renal tubular epithelial damage, and the recovery of renal function depends on renal tubular epithelial cell regeneration. In the event of renal tubular necrosis, blood purification is the only method that can maintain a stable internal bodily environment due to the impairment of kidney function. Major Science and Technology Project (2018ZF007) and the Yunnan Province Applied Basic Research Program Key Project (2018FA041, 2017FA040). National Natural Science Foundation (31970515), 920th Hospital of the PLA Joint Logistics Support Force Inhospital technology plans (2019YGB17, 2019YGA05). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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