Abstract
Objective To study curative effects and mechanism of bone marrow stromal cells (BMSCs) transplantation on rat models of Parkinson's disease (PD). Methods BMSCs were induced into TH-positive cells, which were stained by immunofluorescence with TH and detected by flow cytometry. The 6-hydroxydopamine (6-OHDA) induced hemiparkinsonian rats were selected and allocated randomly into 3 greups,which were. BMSCs labeled with brdu induced into TH positive cells (1.2×106 cells/each model), BMSCs without induced and physiological saline (12 μl) were injected into right striatum of PD rat respectively. The rotation test was taken at 6 week after transplantation. Brain frozen sections were car-fled out immunofluorescence double staining (BrdU/GFAP,BrdU/NSE and BrdU/TH) at 6 week of BM-SCs. Meanwhile the level of dopamine in nigrostriatal system were observed by High-performance liquid chromatography. Results The percentage of TH-positive cells was 10.3% by detecting with flow cytome-try. Frequency of changed rotation were significantly more in induced than non-induced BMSCs group and normal saline group at 6 week after transplantation. Some BrdU/NSE, BrdU/TH positive cells could be found in the implanted region of BMSCs induction group at 6 week after transplantation. At this time, Br-dU/NSE and BrdU/TH positive cells are not found in the implanted region of BMSCs group but there are BrdU/GFAP positive cells, the level of dopamine in nigrostriatal system in the implanted region of BMSCs induction group at 6 week after transplantation, were more than BMSCs group observed by High-perform-ance liquid chromatography. Conclusion BMSCs induced into TH-positive cells had better effects on treating PD rat models than that of BMSCs without induced group, and physiological saline group. The reasearch provicled the evideme of treating PD. Key words: Parkiason disease; Bone marrow stromal cells; Dopaminergic neurons; Trans-plantation
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