Transplantation of autologous angiogenin-overexpressing marrow stromal cells in treatment of a porcine chronic ischemia model

Abstract

Objective To investigate the effect of autologous angiogenin(ANG)-overexpressing marrow stromal cells transplantation on the neoangiogenesis and cardiac function in a porcine model of chronic ischemia.Methods Porcine models of chronic ischemia were established and were randomized into the following 3groups:autologous marrow stromal cells transfected with Ad-ANG(GroupⅠ,n=11),autologous marrow stromal cells without transfection(GroupⅡ,n=10),and controls injected with serum-free DMEM medium(GroupⅢ,n=10).The autologous marrow stromal cells were cultured and were transfected with Ad-ANG,and the cells were labeled by CM-DiI.The cells were transplanted to the ischemia site of porcine heart.The parameters we observed included Rentrop score,ejection fraction,percentage of infracted area,CM-DiI labeled cells under fluorescence microscope,number of blood vessels and expression of ANG protein.Results Compared with groupⅡandⅢ,GroupⅠ had significantly increased Rentrop score,ejection fraction,numbers of blood vessels in the infarcted areas,CM-DiI labeled cells,and ANG protein expression,but significantly decreased infarcted area(P0.05or P0.01).Conclusion Transplantation of autologous angiogenin-overexpressing marrow stromal cells can yield a high survival rate of cells,greatly improving the blood supply of the infarcted cardiac area and cardiac function in porcine model.