Abstract
Landschütz ascites tumour cells exposed in vitro to neuraminidase (VCN) were tested for viability, sialic acid depletion and subsequent tumour development in three mouse strains. Treatment with 25 U VCN/ 10 7 cells/ml released 50% of the total cellular sialic acid content and no further release was found using a 20 fold higher enzyme concentration at pH 5.5. The treated cells were normally viable as assessed by vital dye exclusion. VCN reduced tumour transplantability at certain inoculum levels but above a particular tumour dose for each mouse strain no such effect was observed. Sera from the three mouse strains used showed no cytotoxic activity in vitro against untreated or VCN-treated cells.
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