Transparent biosensor with micro channel array for optical and electrophysiological detection of luciferin–luciferase reaction

Abstract

AbstractThis paper describes a transparent biosensor that was designed to detect both the optical and electrophysiological signals of the luciferin–luciferase reaction. By using microelectromechanical system (MEMS) technologies, we have studied and developed a ‘Micro Channel Array’ (MCA) integrated with suction holes and electrodes as planar patch‐clamp biosensors. A transparent biosensor was developed as an advanced MCA. The developed transparent biosensor employs an SU‐8‐based MCA structure for simultaneous multipoint sensing of optical and electrophysiological signals. In this study, we tested the newly developed biosensor using the mPer1‐luc slice culture, which has circadian rhythms and is a typical example of cells in which a luciferin–luciferase reaction occurs. We could measure the optical and electrophysiological signals of the mPer1‐luc slice culture. Here we describe the experimental results of this study, and describe the design and fabrication of the transparent biosensor with MCA. Copyright © 2007 Institute of Electrical Engineers of Japan© 2007 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.