Transovarial transmission of dengue virus on Aedes aegypti atseveral endemic areas of dengue haemorrhagic fever in Indonesia

Abstract

Transovarial transmission of dengue virus (DENV) on Aedes aegypti is reported as other mechanisms that played a role in the transmission of dengue hemorrhagic fever (DHF). The aim of research to determine the prevalence of vertical transmission in Ae. aegypti from in several endemic areas of dengue in Indonesia. Sample of mosquitoes were collected from at least 25 locations in West Java and 29 locations outer of West Java (Palembang South Sumatra, Banda Aceh, Lombok West Nusa Tenggara, and Ternate Island) by using ovitraps. Then eggs obtained were hatched in the laboratory to be larvae and become adults. The virus detection from the adult specimens were done by using RT-PCR. The results showed the DENV transovarial transmission in Ae. aegypti in three endemic areas of dengue in West Java (25 isolates), for a while still negatives because the mosquitoes from the area Bogor, Sukabumi and Tasikmalaya examined were not found to contain DENV. Meanwhile, of the 29 isolates in the outer West Java (Palembang South Sumatra, Banda Aceh, Lombok West Nusa Tenggara, and Ternate Island), 8 of themwere detected to have DENV-2. DENV transovarial transmission were not be detectedin West Java because of many factors, such as the nature of DENV was very labile to temperature, humidity and chemical factors, as well as a short period of viremia that affect the success rate of DENV transmission to Ae. aegypti.