Abstract
The alpha herpesviruses have evolved to enter the peripheral nervous systems of their natural hosts and establish a quiescent infection (latency) in peripheral ganglia that can be reactivated at a later time. The spread of infection is through chains of synaptically connected neurons. The biology of pseudorabies virus (PRV), a swine herpesvirus with a very broad host range (essentially all mammals except higher primates), is of some interest for several reasons. In all of its hosts except swine, PRV infection is neuroinvasive and lethal. In addition, certain attenuated mutants can spread only in one direction in chains of synaptically connected neurons. These strains are used in tracing neural circuitry in a variety of mammals and bird embryos. The viral genome inside capsids is moved long distances in axons in a controlled fashion on microtubules. Sorting of virion components into axons controls the directional, neuronal spread of infection of alpha herpesviruses. We have been studying two aspects of the general problem. First, using GFP and mRFP fusion proteins, we have used video confocal microscopy to assess mechanisms and gene products that influence spread of Pseudorabies (PRV) virion components within axons. Second, we have developed an in vitro cell culture system that enables us for the first time, to study axon-mediated, transneuronal spread from neurons to neurons as well as neuron to non-neuronal cells similar to what happens after reactivation and spread to epithelial surfaces. These systems appear to duplicate all the known in vivo correlates of transneuronal spread and provide the opportunity to do both quantitative and qualitative assessment of spread of PRV infection. Funding NIH RO133506, NCRR P40 RRO1 18604 from Infectious diseases of the nervous system: pathogenesis and worldwide impact Paris, France. 10–13 September 2008
Highlights
Transneuronal spread of alpha herpesvirus infection: lessons learned from using pseudorabies virus (PRV) as a neural circuit tracer
Infectious diseases of the nervous system: pathogenesis and worldwide impact Roberto Bruzzone, Monique Dubois-Dalcq, Georges E Grau, Diane E Griffin and Krister Kristensson Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here.
The alpha herpesviruses have evolved to enter the peripheral nervous systems of their natural hosts and establish a quiescent infection in peripheral ganglia that can be reactivated at a later time
Summary
Infectious diseases of the nervous system: pathogenesis and worldwide impact Roberto Bruzzone, Monique Dubois-Dalcq, Georges E Grau, Diane E Griffin and Krister Kristensson Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. . Transneuronal spread of alpha herpesvirus infection: lessons learned from using PRV as a neural circuit tracer Address: Department of Molecular Biology, Princeton University, Princeton, New Jersey, 08544, USA Email: Lynn W Enquist - Lenquist@princeton.edu from Infectious diseases of the nervous system: pathogenesis and worldwide impact Paris, France.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have