Abstract
Milo strains of the tristeza virus of citrus have been transmitted experimentally by several species of aphids. Recent tests show that the melon aphid ( Aphis gossypii Glov.) is also capable of transmitting the severe T3 strain of this virus. The sources of inoculum were young potted pineapple orange, Cleopatra mandarin, and rough lemon seedlings grown under greenhouse conditions and inoculated with the T3 strain by means of tissue grafts. These plants were tested for presence of this strain following inoculation by tissue grafts to healthy Key lime indicator plants. Large numbers of these aphids were fed on the source plants for 24 hours, and then were transferred in colonies averaging 300 to Key lime indicator plants for a minimum of 6 hours for transmission feeding. Symptoms Characteristic of the T3 strain of tristeza appeared on 11 of 16 indicator plants in 94 days.
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