Abstract
BackgroundHuman enterovirus 85 (HEV85), whose prototype strain (Strain BAN00-10353/BAN/2000) was isolated in Bangladesh in 2000, is a recently identified serotype within the human enterovirus B (HEV-B) species. At present, only one nucleotide sequence of HEV85 (the complete genome sequence of the prototype strain) is available in the GenBank database.Principal FindingsIn this study, we report the genetic characteristics of 33 HEV85 isolates that circulated in the Xinjiang Uighur autonomous region of China in 2011. Sequence analysis revealed that all these Chinese HEV85 isolates belong to 2 transmission chains, and intertypic recombination was found with the new unknown serotype HEV-B donor sequences. Two HEV85 isolates recovered from a patient presenting acute flaccid paralysis and one of his contacts were temperature-insensitive strains, and some nucleotide substitutions in the non-coding regions and in the 2C or 3D coding regions may have affected the temperature sensitivity of HEV85 strains.ConclusionsThe Chinese HEV85 recombinant described in this study trapped a new unknown serotype HEV-B donor sequence, indicating that new unknown HEV-B serotypes exist or circulate in Xinjiang of China. Our study also indicated that HEV85 is a prevalent and common enterovirus serotype in Xinjiang.
Highlights
Human enteroviruses (HEV) are members of the genus Enterovirus within the family Picornaviridae, order Picornavirales, and contains 4 species: HEV-A, human enterovirus B (HEV-B), HEV-C, and HEV-D [1]
The Chinese Human enterovirus 85 (HEV85) recombinant described in this study trapped a new unknown serotype HEV-B donor sequence, indicating that new unknown HEV-B serotypes exist or circulate in Xinjiang of China
Our study indicated that HEV85 is a prevalent and common enterovirus serotype in Xinjiang
Summary
Human enteroviruses (HEV) are members of the genus Enterovirus within the family Picornaviridae, order Picornavirales, and contains 4 species: HEV-A, HEV-B, HEV-C, and HEV-D [1]. The genome of HEV (approximately 7,450 nucleotides) is positive-sense, single-stranded, and contains a long open reading frame (ORF) flanked by a 59-untranslated region (UTR) and a 39UTR. The 59-UTR is about 740 nucleotides in length and comprises a secondary structure named the internal ribosome entry site [18] which is involved in the replication and internal initiation of translation of the genomic RNA [19]. A short 39-UTR, approximately 100 nucleotides in length, is located between the ORF and the poly (A) stretch, and comprises structural domains involved in RNA replication [20]. Only one nucleotide sequence of HEV85 (the complete genome sequence of the prototype strain) is available in the GenBank database
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