Transmission electron microscopy and immunohistoc hemical studies on the effect of platelet rich fibrin at the bone- metallic interface in the mandible

Abstract

Background: A dental implant is an effective method for restoring edentulous dentition. Manybiological materials were used to coat implant surfaces to enhance osseointegration. Growth factorslike bone morphogenic proteins (BMP) were used for this purpose. Platelet rich fibrin (PRF ) is anew approach on bone regeneration through the synergistic effect of many growth factors derivedfrom platelets.Purpose: The purpose of this study was the evaluation of the effect of platelet rich fibrin (PRF )and transforming growth factor (TGF β) on the bone- metallic interface in dogs. Also, to determinethe relationship between titanium implants and bone cells by the electron microscopy.Methods & materials: Six healthy adult male dogs included in this study. The dogs weredivided randomly into two groups. The dog’s mandibles were used. The third premolar wasextracted bilaterally and the right side used as a control while the left used as experimental side. PRFwas prepared and the immediate implant was inserted. Ultrastructure and immunohistochemicalexamination of dogs were evaluated.Results: The results demonstrated that the PRF and TGF β enhance the osseointegration in theexperimental group when compared with control group. During study of bone regeneration moreadvanced intervals were required for bone maturation.Conclusions: The PRF accelerates early bone regeneration and there is a tight regulatoryrelationship between the TGF β and the rapidity of bone formation.