Translokation bakterieller Effektorproteine — live und in Farbe

Abstract

Intracellular pathogens thrive by invading host cells, thereby creating protected replicative niches. The manipulation of host cells is accomplished by means of translocated bacterial effector proteins. The ability to track effector proteins during an infection is essential to understand host-pathogen interaction. Here, we describe a novel method to target bac terial effector proteins using Salmonella enterica as a model organism and deploying self-labeling enzyme tags.