Abstract
Cross-linking of receptor bound IgE antibodies by multivalent antigen (DNP 8-BSA) on PB-3c cells leads to an increase of cytosolic calcium ((Ca 2+) i). Active tumor promoting phorbol esters and teleocidin which specifically activate the phospholipid Ca 2+-sensitive protein kinase (PKC), inhibited the antigen-medicated rise in (Ca 2+) i and induced a time and dose-dependent translocation of cytosolic PKC to membranes of the PB-3c cells as determined by enzyme activity or immunoblotting using a polyclonal anti-PKC antibody. This TPA concentration did not affect the subcellular distribution of PKC, although 1nM of 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibited to 50% the antigen-mediated increase in (Ca 2+) i. The concentration of TPA required to induce a half-maximal subcellular redistribution of immunodetactable PKC activity was an order of magnitude greater than the half-maximal dose required to inhibit the antigen-mediated increase in (Ca 2+) i. These data demonstrate that the TPA-dependent activation of PKC is not directly coupled to its translocation to membranes.
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