Translation Products of RNA from Indian Peanut Clump Virus

Abstract

SUMMARY When RNA from particles of Indian peanut clump virus (IPCV) was translated using messenger-dependent reticulocyte lysate the largest product had a mol. wt. of about 195000 and the other main products had mol. wt. of 143000 and 24500. The 24500 mol. wt. polypeptide co-migrated with IPCV coat protein and was the only product that reacted with antiserum to IPCV particles. Translation of separated RNA species showed that the 195000 mol. wt. and 143000 mol. wt., as well as some smaller less abundant products, arose from translation of RNA-1 (mol. wt. 1.9 × 106) whereas RNA-2 (mol. wt. 1.6 × 106) was translated to give only the 24500 mol. wt. product Translation of RNA of mol. wt. 0.5 × 106 to 0.9 × 106 gave a 50000 mol. wt. product that was present and sometimes prominent in translation products of unfractionated RNA. A product of about 20000 mol. wt. was made by translation of RNA of about 0.2 × 106 mol. wt. IPCV resembles the viruses with bipartite genomes whose larger RNA species is translated into large polypeptides and whose coat protein gene is on the smaller RNA species. The other translation products may be those of subgenomic messenger, or of satellite, RNA species.