Abstract
Total messenger RNA was extracted from a human T cell hybridoma, clone H-E4-9, which strongly produced macrophage activating factor for glucose consumption (MAF-G). This messenger RNA gave rise to functional MAF-G when translated in Xenopus laevis oocytes. Isoelectric focusing of culture supernatants of the mRNA-microinjected oocytes and the H-E4-9 cells revealed that the former contained MAF-Gs with isoelectric points of pH 5.0 and 3.0 while the latter contained MAF-Gs with isoelectric points of pH 5.0 and pH 3.3. Sucrose density gradient centrifugation analysis showed that MAF-G mRNA prepared from H-E4-9 cells sedimented at about 11.5 S.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
