Translation Initiation Factor eIF4E and eIFiso4E Are Both Required for Peanut stripe virus Infection in Peanut (Arachis hypogaea L.).

Manlin Xu,Jinguang Yang,Yucheng Chi,Juxiang Wu,Hongfeng Xie,Lianhui Xie

doi:10.3389/fmicb.2017.00338

Abstract

Peanut stripe virus (PStV) belongs to the genus Potyvirus and is the most important viral pathogen of cultivated peanut (Arachis hypogaea L.). The eukaryotic translation initiation factor, eIF4E, and its isoform, eIF(iso)4E, play key roles during virus infection in plants, particularly Potyvirus. In the present study, we cloned the eIF4E and eIF(iso)4E homologs in peanut and named these as PeaeIF4E and PeaeIF(iso)4E, respectively. Quantitative real-time PCR (qRT-PCR) analysis showed that these two genes were expressed during all growth periods and in all peanut organs, but were especially abundant in young leaves and roots. These also had similar expression levels. Yeast two-hybrid analysis showed that PStV multifunctional helper component proteinase (HC-Pro) and viral protein genome-linked (VPg) both interacted with PeaeIF4E and PeaeIF(iso)4E. Bimolecular fluorescence complementation assay showed that there was an interaction between HC-Pro and PeaeIF4E/PeaeIF(iso)4E in the cytoplasm and between VPg and PeaeIF4E/PeaeIF(iso)4E in the nucleus. Silencing either PeaeIF4E or PeaeIF(iso)4E using a virus-induced gene silencing system did not significantly affect PStV accumulation. However, silencing both PeaeIF4E and PeaeIF(iso)4E genes significantly weakened PStV accumulation. The findings of the present study suggest that PeaeIF4E and PeaeIF(iso)4E play important roles in the PStV infection cycle and may potentially contribute to PStV resistance.

Highlights

Peanut is one of the most important oil crops and food legumes in the world
The product with the correct sequence eIF4E and eIFiso4E Are Required for Peanut stripe virus Infection was used in designing primers (Supplementary Table S1) for 5 amplification of cDNA ends (5 -RACE) and 3 -RACE to obtain the full-length cDNA of PeaeIF4E
The cloned peanut eIF4E and eIF(iso)4E genes were designated as peaeIF4E and peaeIF(iso)4E, respectively

Summary

Introduction

In China, peanuts are grown on 3.5 million hectares of land each year (Xu, 2008). PStV has been detected in various countries, including China, the US, the Philippines, Thailand, Indonesia, Malaysia, and Korea (Xu et al, 1983; Demski and Lovell, 1985; Saleh et al, 1989; Choi et al, 2001, 2006). EIF4E and eIFiso4E Are Required for Peanut stripe virus Infection. PStV has infected various other crops, including soybean (Glycine max), sesame (Sesamum indicum), cowpea (Vigna unguiculata), hyacinth bean (Dolichos lablab), white lupin (Lupinus albus), and patchouli (Pogostemon cablin; Xu, 2008; Singh et al, 2009). No effective method for controlling this virus has been established

Methods

Results

Conclusion