Abstract
We report RNA self-replication through the translation of its encoded protein within membrane-free compartments generated by liquid-liquid phase separation. The aqueous droplets support RNA self-replication by concentrating a genomic RNA and translation proteins, facilitating the uptake of small substrates, and preventing the replication of parasitic RNAs through compartmentalization.
Highlights
We report RNA self-replication through the translation of its encoded protein within membrane-free compartments generated by liquid–liquid phase separation
We further show additional roles of aqueous twophase systems (ATPS) in facilitating the sustained replication of the genomic RNA: the prevention of parasitic RNA replication and the facile uptake of building blocks
The translation-coupled RNA replication (TcRR) system consists of an artificial single-stranded genomic RNA (2041 nt) that encodes the core subunit of Qb replicase and a reconstituted E.coli translation system based on the PURE
Summary
We report RNA self-replication through the translation of its encoded protein within membrane-free compartments generated by liquid–liquid phase separation. The aqueous droplets support RNA self-replication by concentrating a genomic RNA and translation proteins, facilitating the uptake of small substrates, and preventing the replication of parasitic RNAs through compartmentalization.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.