Abstract
We report RNA self-replication through the translation of its encoded protein within membrane-free compartments generated by liquid-liquid phase separation. The aqueous droplets support RNA self-replication by concentrating a genomic RNA and translation proteins, facilitating the uptake of small substrates, and preventing the replication of parasitic RNAs through compartmentalization.
Highlights
We report RNA self-replication through the translation of its encoded protein within membrane-free compartments generated by liquid–liquid phase separation
We further show additional roles of aqueous twophase systems (ATPS) in facilitating the sustained replication of the genomic RNA: the prevention of parasitic RNA replication and the facile uptake of building blocks
The translation-coupled RNA replication (TcRR) system consists of an artificial single-stranded genomic RNA (2041 nt) that encodes the core subunit of Qb replicase and a reconstituted E.coli translation system based on the PURE
Summary
We report RNA self-replication through the translation of its encoded protein within membrane-free compartments generated by liquid–liquid phase separation. The aqueous droplets support RNA self-replication by concentrating a genomic RNA and translation proteins, facilitating the uptake of small substrates, and preventing the replication of parasitic RNAs through compartmentalization.
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